lenticrisprv2 puro vector (Addgene inc)
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Addgene inc
lenticrisprv2 puro vector
Lenticrisprv2 Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+vectors/lentiCRISPRv2+puro+(Plasmid+%2398290)/pmc13007490-34-8-10
Average 98 stars, based on 516 article reviews
Lenticrisprv2 Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+vectors/lentiCRISPRv2+puro+(Plasmid+%2398290)/pmc13007490-34-8-10
Average 98 stars, based on 516 article reviews
lenticrisprv2 puro vector - by Bioz Stars,
2026-09
98/100 stars
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Knock-Out:Article Title: Targeting tumor-intrinsic CDK1/Cyclin B1 complex improves responses to immunotherapy in pancreatic cancer. Article Snippet: Immunotherapies, especially immune checkpoint blockade, has achieved considerable success in multiple cancers; however, patients with “cold tumors”, in particular pancreatic cancer, rarely respond, largely due to insufficient T cell activation and infiltration within the tumor microenvironment (TME).. To understand the mechanisms whereby tumor cells regulate T cell activation, we conducted an in vivo CRISPR-Cas9 screen, and identified the CDK1/Cyclin B1 complex as a previously unrecognized tumor-intrinsic driver of immune evasion in pancreatic cancer.. Genetic ablation or pharmacological inhibition of the CDK1/Cyclin B1 complex induced a T cell-inflamed TME, which synergized with PD-1 blockade to suppress tumor growth. Clone Assay:Article Title: Targeting tumor-intrinsic CDK1/Cyclin B1 complex improves responses to immunotherapy in pancreatic cancer. Article Snippet: Immunotherapies, especially immune checkpoint blockade, has achieved considerable success in multiple cancers; however, patients with “cold tumors”, in particular pancreatic cancer, rarely respond, largely due to insufficient T cell activation and infiltration within the tumor microenvironment (TME).. To understand the mechanisms whereby tumor cells regulate T cell activation, we conducted an in vivo CRISPR-Cas9 screen, and identified the CDK1/Cyclin B1 complex as a previously unrecognized tumor-intrinsic driver of immune evasion in pancreatic cancer.. Genetic ablation or pharmacological inhibition of the CDK1/Cyclin B1 complex induced a T cell-inflamed TME, which synergized with PD-1 blockade to suppress tumor growth. Article Title: Calneuron 1 reveals the pivotal roles in schizophrenia via perturbing human forebrain development and causing hallucination-like behavior in mice Article Snippet: .. Each gRNA was cloned into the Selection:Article Title: Targeting tumor-intrinsic CDK1/Cyclin B1 complex improves responses to immunotherapy in pancreatic cancer. Article Snippet: Immunotherapies, especially immune checkpoint blockade, has achieved considerable success in multiple cancers; however, patients with “cold tumors”, in particular pancreatic cancer, rarely respond, largely due to insufficient T cell activation and infiltration within the tumor microenvironment (TME).. To understand the mechanisms whereby tumor cells regulate T cell activation, we conducted an in vivo CRISPR-Cas9 screen, and identified the CDK1/Cyclin B1 complex as a previously unrecognized tumor-intrinsic driver of immune evasion in pancreatic cancer.. Genetic ablation or pharmacological inhibition of the CDK1/Cyclin B1 complex induced a T cell-inflamed TME, which synergized with PD-1 blockade to suppress tumor growth. Article Title: Integrated thermal proteome and thermal proximity co-aggregation profiling identifies ATP6V1C1 as a novel anti-cancer drug target Article Snippet: .. Produced:Article Title: Tissue factor-induced fibrinogenesis mediates cancer cell clustering and multiclonal peritoneal metastasis. Article Snippet: 293FT cells were purchased from Thermo Fisher Scientific and maintained in DMEM high glucose (Sigma-Aldrich) supplemented with 10% FBS, 0.1 mM MEM Non-Essential Amino Acids, 1 mM sodium pyruvate, 2 mM L-glutamine, 500 μg/mL G418, and antibiotics. .. Lentiviral particles were produced by transfecting Lenti-X 293T cells (Takara Bio) with ViraPower (Thermo Fisher Scientific) or psPAX2 and pMD2.G (gifts from Didier Trono, Addgene Plasmid #12260 and #12259, respectively) together with lentiviral constructs, including those generated using the pCDH-EF1-MCS-IRES-Neo/Puro (System Biosciences) and Plasmid Preparation:Article Title: Tissue factor-induced fibrinogenesis mediates cancer cell clustering and multiclonal peritoneal metastasis. Article Snippet: 293FT cells were purchased from Thermo Fisher Scientific and maintained in DMEM high glucose (Sigma-Aldrich) supplemented with 10% FBS, 0.1 mM MEM Non-Essential Amino Acids, 1 mM sodium pyruvate, 2 mM L-glutamine, 500 μg/mL G418, and antibiotics. .. Lentiviral particles were produced by transfecting Lenti-X 293T cells (Takara Bio) with ViraPower (Thermo Fisher Scientific) or psPAX2 and pMD2.G (gifts from Didier Trono, Addgene Plasmid #12260 and #12259, respectively) together with lentiviral constructs, including those generated using the pCDH-EF1-MCS-IRES-Neo/Puro (System Biosciences) and Article Title: Osteopontin monoclonal antibodies Article Snippet: SgRNAs specific to Spp1 (5′-AAGGTGAAAGTGACTGATTC-3′ (SEQ ID NO:46)) or a control sequence not present in the mouse genome (5′-CTCGTATCTTTTCCCACGGC-3′ (SEQ ID NO:47)) were purchased in the lentiCRISPRv2-Puro vector (Genscript, Piscataway). .. HEK293FT cells at 60-70% confluency were co-transfected with indicated Construct:Article Title: Tissue factor-induced fibrinogenesis mediates cancer cell clustering and multiclonal peritoneal metastasis. Article Snippet: 293FT cells were purchased from Thermo Fisher Scientific and maintained in DMEM high glucose (Sigma-Aldrich) supplemented with 10% FBS, 0.1 mM MEM Non-Essential Amino Acids, 1 mM sodium pyruvate, 2 mM L-glutamine, 500 μg/mL G418, and antibiotics. .. Lentiviral particles were produced by transfecting Lenti-X 293T cells (Takara Bio) with ViraPower (Thermo Fisher Scientific) or psPAX2 and pMD2.G (gifts from Didier Trono, Addgene Plasmid #12260 and #12259, respectively) together with lentiviral constructs, including those generated using the pCDH-EF1-MCS-IRES-Neo/Puro (System Biosciences) and Generated:Article Title: Tissue factor-induced fibrinogenesis mediates cancer cell clustering and multiclonal peritoneal metastasis. Article Snippet: 293FT cells were purchased from Thermo Fisher Scientific and maintained in DMEM high glucose (Sigma-Aldrich) supplemented with 10% FBS, 0.1 mM MEM Non-Essential Amino Acids, 1 mM sodium pyruvate, 2 mM L-glutamine, 500 μg/mL G418, and antibiotics. .. Lentiviral particles were produced by transfecting Lenti-X 293T cells (Takara Bio) with ViraPower (Thermo Fisher Scientific) or psPAX2 and pMD2.G (gifts from Didier Trono, Addgene Plasmid #12260 and #12259, respectively) together with lentiviral constructs, including those generated using the pCDH-EF1-MCS-IRES-Neo/Puro (System Biosciences) and Sequencing:Article Title: Calneuron 1 reveals the pivotal roles in schizophrenia via perturbing human forebrain development and causing hallucination-like behavior in mice Article Snippet: .. Each gRNA was cloned into the |